Chiral Ligands
Novel Biphenyl Chiral Ligands
Biaryl Ligands Developed by Our Group
Ligands wtih D2 Symmetric Backbone
The development of TOX
The development of TOH
The development of DIP
Ligands wtih Wider Range of Dihedral Angle
Tropos to Atrops: BriP
The development of BrOx and BrinP
Tropos Ligands
The development of BiphOx
The development of BPPHOX
Organocatalysis
Novel Nucleophilic Organocatalyst
Asymmetric Reactions
Asymmetric Allylic Substitution
Enamines as Nucleophilic Reagents
Enamines Generated in situ
Allylic C-N Cleavage under Mild Conditions
Wacker-type Oxidative Cyclization
Pd-Quinox Catalyzed Aza-Wacker Type Cyclization
Switchable Regioselective Pd-Catalyzed Aerobic Aza-Wacker Cyclization--Ligands effect
Allylic C-N Cleavage under Mild Conditions
Asymmetric Hydrogenation
Asymmetric Organocatalyses
Process Research
Fundations
Doxycycline (doxycycline hyclate, doxycycline
hydrochloride or Dox), a synthetic tetracycline
(Tc) derivative, is the effector molecule for
Clontech's Tet-On® and Tet-Off® Inducible
Expression Systems. Dox can be used at
100-fold lower concentrations than Tc,
yielding inducing concentrations ranging
from 1ng/ml to 1 µg/ml. In the Tet-On System,
addition of Dox to culture medium induces a
conformational change in the transactivator
which allows it to bind to tet operator
sequences located in the inducible promoter.
This results in transcriptional activation of the
gene of interest. In the Tet-Off System, removal
of Dox from culture medium induces binding
of the transactivator, resulting in transcription
of the gene of interest.